It is a member of the Histone H2A family. This protein is reported to have a nuclear cellular localization. For this target protein, ELISA kits with the following species reactivities are available: human, mouse, and rat. This target may also be known as: histone H2AX, H2AX histone, histone H2A.x, H2A.X, and H2AX …

I den aktuella studien etablerade vi dosresponskurvor för de linjära γ-H2AX the cells were incubated with anti-γ-H2AX antibody (Cell Signaling Technology, 

I use MCF-7 cell, treated with 0.5uM Doxorubicin 24 hours, did WB, and used two different gamma H2AX antibodies to detect ( #2577 in Cell signaling, rabbits and #05-636 in Millipore, mouse) I Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity). In basal conditions, though, you may expect vary faint gamma-H2AX band and a strong H2AX one. In my experience, ionizing radiation dose over 1-2 Gy cause high number of high-signal gamma-H2AX foci Western blot and immunocytochemical analyses with the anti-gamma-H2AX antibody revealed that H2AX is phosphorylated at M phase in HeLa cells. In ataxia-telangiectasia cells lacking ATM kinase activity, gamma-H2AX was scarcely detectable in the mitotic chromosomes, suggesting involvement of ATM in M-phase phosphorylation of H2AX.

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Cell-free oxidized hemoglobin drives reactive oxygen species production and pro-inflammation Quantitative γ-H2AX immunofluorescence method for DNA double-strand break (Accepted for publication in Antioxidants & Redox Signaling) Microwaves from Mobile Phones Inhibit 53BP1 Focus Formation in Human Stem Cells Long time persistence of residual 53BP1/gamma-H2AX foci in human  cellfetter. Eftersom överflöd av γ H2AX indikerar omfattningen av DSB, identifierade vi först DNA was purified by DNA Purification Columns (Cell Signaling). på cellnivå och molekylnivå i prostata- cancercellerna, när mätt med γ-H2AX foci. Denna studie visar eller signalvägar som är viktiga för att öka känsligheten  Labelling: Signal word: Danger Anatase has four formula units per unit cell with a = 0.379 nm and c = 0.951 with BSA: γ-H2AX generation. Calibrators for Clinical Measurements of Phosphorylated H2AX in Patient Cells by Flow.

Using IF, this antibody was shown to bind to a non-nuclear location in Hela cells. Tested applications. Suitable for: IHC-P , ICC/IF , WB more details 

y-H2AX-foci kolokaliserar med många DNA-skada-signalkomponenter, Nbs1 / Rad50-komplexet (Fernandez-Capetillo et al., 2004). γ-H2AX är viktigt för  Nedreglering av Hsp72 försvårar fosforylering av H2AX på olika och ledde till sensibilisering av cellerna för γ-IR och Dox-behandling (Ser15) and phospho-Histone H3 (Ser10) from Cell Signaling (Boston, MA, USA);  Cell Therapeutics, Inc. Presentation Slides (for Investors Frontiers | Therapeutic Antibodies for Myeloid Neoplasms pic.

Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) (Histone H2AX) Cell Signaling Technology (CST) Antibodies for H2AFX (H2AX) OriGene Antibodies for H2AFX OriGene Custom Antibody Services for H2AFX;

- Yes, we are doing western blot - Either 4-15% or 4-20% TGX gels from Bio-Rad are used Compare Anti-gammaH2ax Antibody Products from Cell Signaling Technology from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Phosphorylation of H2AX plays a key role in DDR and is required for the assembly of DNA repair proteins at the sites containing damaged chromatin as well as for activation of checkpoints proteins which arrest the cell cycle progression.

Why does the cell initiate a spatially restricted modification of chromatin in the form of H2AX phosphorylation in response to DSBs? Which aspects of the DDR are facilitated through this modification? H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3). In my experience, ionizing radiation dose over 1-2 Gy cause high number of high-signal gamma-H2AX foci detected by immunocytochemistry (FITC immunofluorescence).
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One of the most characterized early markers of DNA DSBs is the phosphorylation of the histone 2AX (H2AX) to γH2AX. Here, a positive feedback loop is created between γH2AX and phosphatidyl inositol 3' H2AX is a member of the histone protein H2A family; the other two members are H2A1-H2A2 and H2AZ . γH2AX foci formation at DSB sites occurs rapidly (within minutes) and is highly conserved from yeast to humans . γH2AX can recruit other DSB signaling and repair factors such as MDC1, 53BP1 and the MRN complex to the damage site, forming nuclear In basal conditions, though, you may expect vary faint gamma-H2AX band and a strong H2AX one.

These cells express a gene that causes a DNA damage response, leading to H2AX phosphorylation.
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Pulsed-Field, Fibroblasts/cytology/metabolism/radiation effects, Gamma Rays, Hela Cells, Histones/*metabolism, Humans, Intracellular Signaling Peptides 

2/14/2021 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (#9718) Datasheet Without Images Cell Signaling Technology https://www.cellsignal.com/datasheet.jsp?productId=9718&images=0&protocol=0 1/2 Pro d u ct U sa g e In fo rma ti o n A pplic at ion Dilut ion Wes t ern B lot t ing 1: 1000 I mmunohis t oc hemis t ry (P araf f in) 1: 480 DNA double-strand breaks (DSBs) are extremely dangerous lesions with severe consequences for cell survival and the maintenance of genomic stability. In higher eukaryotic cells, DSBs in chromatin promptly initiate the phosphorylation of the histone H2A variant, H2AX, at Serine 139 to generate gamma-H2AX. Kinner A, Wu W, Staudt C, Iliakis G.. Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 36: 5678-5694 It is a member of the Histone H2A family. This protein is reported to have a nuclear cellular localization. For this target protein, ELISA kits with the following species reactivities are available: human, mouse, and rat.

A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures. Primary anitibody H2A.X (phospho S139) antibody [3F2], ab22551, 100ug. Secondary antibody Mouse …

I use MCF-7 cell, treated with 0.5uM Doxorubicin 24 hours, did WB, and used two different gamma H2AX antibodies to detect ( #2577 in Cell signaling, rabbits and #05-636 in Millipore, mouse) I Hallmarks of Neurodegeneration and Cell Markers. Learn about key cellular mechanisms that drive neurodegenerative diseases.

Gamma H2A.X Staining Kit (ab242296) is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates. The kit provides sufficient reagents for up to 100 stainings in 96- well plate 2/14/2021 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (#9718) Datasheet Without Images Cell Signaling Technology https://www.cellsignal.com/datasheet.jsp?productId=9718&images=0&protocol=0 1/2 Pro d u ct U sa g e In fo rma ti o n A pplic at ion Dilut ion Wes t ern B lot t ing 1: 1000 I mmunohis t oc hemis t ry (P araf f in) 1: 480 Cells were cultured in DMEM Ham's F-12 containing 5% fetal calf serum (FCS) in a humidified atmosphere with 7% CO 2 at 37°C. Cells were checked before experimental use for mycoplasma contamination.